Antibacterial efficacy of pineapple (Ananas Comosus) peels extracts on some selected clinical enterobacteriaceae

Abstract

The antibacterial efficacy of extracts from Pineapple (Ananas comosus) peels carried out on some selected clinical enterobacteriaceae (Salmonella typhi and Shigella dysenteriae) using agar "well" diffusion techniques. The extracts were obtained using two different solvents, ethanol, warm water and fresh juice extract through blending, to obtain the ethanol, aqueous and the fresh juice extracts respectively. The varying concentrations (50, 70, and 90mg/ml) of the crude extracts was used to determine the efficacy of the extracts on the test enterobacteriaceae. The extracts indicate the presence of appreciable compounds such as alkaloids, flavonoids, tannins and cardiac glycosides while glycosides and steroid were absent. The antibacterial susceptibility test on the crude extracts indicates high efficacy of 20.mm at the highest concentration of 90mg/ml for both ethanol and aqueous extracts while the juice extract did not show any efficacy on the test enterobacteriaceae. The chloramphenicol control used indicates activity of 22.0mm against the two test bacteria S typhi and S dysenteriae. The minimum inhibitory concentration and minimum bacteriocidal concentration indicates the plant extracts to be highly potent on the test enterobacteriaceae except the fresh juice extracts fails to show any potency on the test enterobacteriaceae. This research has given a clue for the possibility of using Pineapple peels for remedy on some enterobacteriaceae.