Contraceptive effect on Serum Glucose and Lipid profile in Alloxan-induced diabetic female Wistar Rat

Abstract

Diabetes mellitus is a metabolic disorder that affects many women of reproductive age. Oral contraceptive pills are now commonly used in millions of women worldwide. The study aim to evaluate the effect of a combined oral contraceptive on the glucose levels and lipid profile in alloxan-induced hyperglycemic rats. Twenty-five female albino Wistar rats weighing 150-200g were divided into five groups(A-E) of five rats each. Animals in group A (control) received distilled water, and Groups B-E were induced into hyperglycemia by intraperitoneal injection of alloxan monohydrate (150mg/kg body weight). Groups (B-D) received distilled water, COCs 0.6mg/kg BW, glibenclamide reference antidiabetic drug) 25mg/kg body weight, for 14 days, 18days, and 22 days, respectively, while those in group E received glibenclamide 25mg/kg BW for 22 days. At the end of the study, all rats were sacrificed, blood sample was collected via cardiac puncture and placed in plane bottles for biochemical analysis, Administration of a COCS significantly (P<0.05) increased blood glucose, a well as lipid profile. This result suggests that diabetic rats administered oral contraceptive pills may be more susceptible to oxidative stress by enhanced depletion of antioxidant and increased lipid peroxidation.