Extended and Cryopreserved Semen of Banaba Native Chicken with Sugarcane Extract for Artificial Insemination

DOI: https://doi.org/10.33003/jobasr

Salifu, A-R. S.

Dichoso, G. A.

Sangel, P. P.

Abstract
The experiment was conducted to test the efficacy of sugarcane extract extender (40% SE+30% dH2O (distilled water) + 30% EY-C (Egg-yolk citrate)) developed in our laboratory and semen cryopreserved with SE extender with 3% glycerol (SEG) in in vivo fertility trial using a practical farm approach. The lactated ringers’ solution (LRS) served as a control. The cryopreserved semen was thawed at room temperature (25-27°C) for 15-20 min for the artificial insemination. Thirty-six (36) 7.5 month old Dekalb white hens with an average weight of 1.65 kg were divided into three groups of 12 hens per group and housed individually in a completely randomized design (CRD). The groups: 1, 2 and 3 were artificially inseminated with LRS extended semen, SW extended semen and SWG cryopreserved semen respectively. A total of 284 eggs were collected and incubated, consisting of 95 (group 1), 89 (group 2) and 100 (group 3). The results showed that, fertility rates of LRS extended semen (60.04 ± 1.83%) and SE extended semen (56.94±2.62%) were significantly (p≤0.05) higher than the cryopreserved semen (25.56±3.22 %). However, hatchability rates were statistically similar at 5% among the treatment means for the LRS (51.26±4.15%), SW (62.02±6.34 %) and SEG (49.31±2.86 %). The cryopreserved semen produced fertile eggs and hatching of normal chicks. The findings of this experiment suggest that the natural extender (sugarcane extract with 3% glycerol) did not show deleterious effect on fertility and hatchability and therefore, has a promising future in the cryopreservation of avian semen. Key words: Cryopreserved Semen, Banaba Native Chicken, Sugarcane Extract, Artificial Insemination.
References
Abouelezz, F. M. K., Castaño, C., Toledano‐Díaz, A., Esteso, M. C., López‐Sebastián, A., Campo, J. L., & Santiago‐Moreno, J. (2015). Effect of the interaction between cryoprotectant concentration and cryopreservation method on frozen/thawed chicken sperm variables. Reproduction in domestic animals, 50(1), 135-141. DOI: https://doi.org/10.1111/rda.12464 Abouelezz, F. M. K., Sayed, M. A. M., & Santiago-Moreno, J. (2017). Fertility disturbances of dimethylacetamide and glycerol in rooster sperm diluents: Discrimination among effects produced pre and post freezing-thawing process. Animal reproduction science, 184, 228-234.DOI: https://doi.org/10.1016/j.anireprosci.2017.07.021 Ansari, M., Zhandi, M., Kohram, H., Zaghari, M., Sadeghi, M., & Sharafi, M. (2017). Improvement of post-thawed sperm quality and fertility of Arian rooster by oral administration of d-aspartic acid. Theriogenology, 92, 69-74. DOI: https://doi.org/10.1016/j.theriogenology.2017.01.014 Baguio, S. S., & Capitan, S. S. (2008). Motility, livability and fertility of cock spermatozoa as influenced by day of collection, dilution and cryopreservation. Philippine Journal of Veterinary Medicine, 45: 109-117. Blesbois, E., & Brillard, J. P. (2007). Specific features of in vivo and in vitro sperm storage in birds. Animal, 1(10), 1472-1481. DOI: https://doi.org/10.1017/S175173110700081X Blesbois, E., Grasseau, I., & Seigneurin, F. (2005). Membrane fluidity and the ability of domestic bird spermatozoa to survive cryopreservation. Reproduction, 129(3), 371-378. DOI: 10.1530/rep.1.00454 Burrows, W. H., & Quinn, J. P. (1937). The collection of spermatozoa from the domestic fowl and turkey. Poultry science, 16(1), 19-24. DOI: https://doi.org/10.3382/ps.0160019 Buss, E. G. (1993). Cryopreservation of rooster sperm. Poultry science, 72(5), 944-954. DOI: https://doi.org/10.3382/ps.0720944 Bustani, G. S., & Baiee, F. H. (2021). Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders. Veterinary World, 14(5), 1220. DOI: 10.14202/vetworld.2021.1220-1233 Chinnadurai, C. (2017). Potential health benefits of sugarcane. Sugarcane biotechnology: Challenges and prospects, 1-12. Çiftci, H. B., & Aygün, A. (2018). Poultry semen cryopreservation technologies. World's Poultry Science Journal, 74(4), 699-710. DOI: https://doi.org/10.1017/S0043933918000673 Daramola, J. O., Adekunle, E. O., Oke, O. E., Onagbesan, O. M., Oyewusi, I. K., & Oyewusi, J. A. (2016). Effects of coconut (Cocos nucifera) water with or without egg-yolk on viability of cryopreserved buck spermatozoa. Animal Reproduction (AR), 13(2), 57-62. DOI: 10.21451/1984-3143-AR724 El-Sheshtawy, R. I., El-Badry, D. A., Gamal, A., El-Nattat, W. S., & Almaaty, A. M. A. (2016). Natural honey as a cryoprotectant to improve Arab stallion post-thawing sperm parameters. Asian Pacific Journal of Reproduction, 5(4), 331-334. DOI: https://doi.org/10.1016/j.apjr.2016.06.004 El-Sheshtawy, R. I., El-Nattat, W. S., & Ali, G. A. D. (2017). Cryopreservation of cattle semen using coconut water extender with different glycerol concentrations. Asian Pacific Journal of Reproduction, 6(6), 279-282. DOI: 10.4103/2305-0500.217343 Islam, M. T. (2017). Sugarcane: Safety Concerns on its Cytogenotoxic Damage, While Hopes as a Source of Anticancer Agents. Research Journal of Food & Nutrition, 1(1), 10-12. Lotfi, S., Mehri, M., Sharafi, M., & Masoudi, R. (2017). Hyaluronic acid improves frozen-thawed sperm quality and fertility potential in rooster. Animal reproduction science, 184, 204-210. DOI: https://doi.org/10.1016/j.anireprosci.2017.07.018 Masoudi, R., Sharafi, M., & Pourazadi, L. (2019). Improvement of rooster semen quality using coenzyme Q10 during cooling storage in the Lake extender. Cryobiology, 88, 87-91. DOI: https://doi.org/10.1016/j.cryobiol.2019.03.003 Mehdipour, M., Kia, H. D., Moghaddam, G., & Hamishehkar, H. (2018). Effect of egg yolk plasma and soybean lecithin on rooster frozen-thawed sperm quality and fertility. Theriogenology, 116, 89-94. DOI: https://doi.org/10.1016/j.theriogenology.2018.05.013Get rights and content Mittal, P. K., Madan, A. K., Sharma, V., Gottam, G. S., & Gupta, B. (2019). Cryopreservation of buffalo bull semen-restriction and expectation: A review. International Journal of Current Microbiology and Applied Sciences, 8(1), 1351-1368. DOI: https://doi.org/10.20546/ijcmas.2019.801.144 Mohan, J., Sharma, S. K., Kolluri, G., & Dhama, K. (2018). History of artificial insemination in poultry, its components and significance. World's Poultry Science Journal, 74(3), 475-488. DOI: https://doi.org/10.1017/S0043933918000430 Roushdy, K., El-Sherbieny, M. A., El-Gany, F., & El-Sayed, M. (2014). Semen cryopreservation for two local chicken strains as a tool for conservation of Egyptian local genetic resources. Egyptian Poultry Science Journal, 34(2), 607-618. Salehi, M., Mahdavi, A. H., Sharafi, M., & Shahverdi, A. (2020). Cryopreservation of rooster semen: Evidence for the epigenetic modifications of thawed sperm. Theriogenology, 142, 15-25. DOI: https://doi.org/10.1016/j.theriogenology.2019.09.030 Santiago-Moreno, J., Bernal, B., Pérez-Cerezales, S., Castaño, C., Toledano-Díaz, A., Esteso, M. C., ... & Blesbois, E. (2019). Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance. PloS one, 14(1), e0209910. DOI: https://doi.org/10.1371/journal.pone.0209910 Seigneurin, F., Grasseau, I., Chapuis, H., & Blesbois, E. (2013). An efficient method of guinea fowl sperm cryopreservation. Poultry science, 92(11), 2988-2996. DOI: https://doi.org/10.3382/ps.2013-03166 Shahverdi, A., Sharafi, M., Gourabi, H., Yekta, A. A., Esmaeili, V., Sharbatoghli, M., ... & Mostafayi, F. (2015). Fertility and flow cytometric evaluations of frozen-thawed rooster semen in cryopreservation medium containing low-density lipoprotein. Theriogenology, 83(1), 78-85. DOI: https://doi.org/10.1016/j.theriogenology.2014.07.044 Shanmugam, M., Kumar, K. P., Mahapatra, R. K., & Laxmi, N. A. (2018). Effect of different cryoprotectants on post-thaw semen parameters and fertility in Nicobari chicken. Indian Journal of Poultry Science 53(2), 208-211. DOI: 10.5958/0974-8180.2018.00040.5 Silva, M. A., Peixoto, G. C. X., Lima, G. L., Bezerra, J. A. B., Campos, L. B., Paiva, A. L. C., ... & Silva, A. R. (2012). Cryopreservation of collared peccaries (Tayassu tajacu) semen using a powdered coconut water (ACP-116c) based extender plus various concentrations of egg yolk and glycerol. Theriogenology,78(3), 605-611. DOI:https://doi.org/10.1016/j.theriogenology.2012.03.006 Thananurak, P., Chuaychu-noo, N., & Vongpralub, T. (2017). Freezability and fertility of Thai native chicken semen in different diluents. The Thai Journal of Veterinary Medicine, 47(4), 551-556. DOI: https://doi.org/10.56808/2985-1130.2869 Thélie, A., Bailliard, A., Seigneurin, F., Zerjal, T., Tixier-Boichard, M., & Blesbois, E. (2019). Chicken semen cryopreservation and use for the restoration of rare genetic resources. Poultry Science, 98(1), 447-455. DOI: https://doi.org/10.3382/ps/pey360.
PDF