There has been study on quality of breast-milk per feeding, exclusive breastfeeding but little or nothing on the effect of stress on milk production. This study was carried out to determine the effect of restraint stress and anxiety on milk production using animal model.20 female and 10 male Wistarrats were used for the study. The female rats were caged with mature males in the ratio 5 females to 2 males for two weeks. On delivery, the female rats were separated into their own cages. Each dam in a cage with adequate water and food. The experimental stressor was restraint stress (RSx1) for one hour on day 21. Rats were restrained in a customized Plexiglas Restrainer for restraint stress. After delivery and prior to exposure to physical restraint stressors, the pups of each dam and the dams were weighed using a digital weighing scale with 0.1g precision. These weights were recorded. The dams from each group were euthanized and their blood samples were collected in a plain test tube for hormonal assay using ELISA technique.The results showed that the rats which were subjected to restraint stress showed an increase in milk production compared to the control group (P≤0.05). In the control group the milk yield is 51.3ng/ml, which was relatively low compared to that of group four (the group that underwent restraint stress for 18 days) which is 291.7ng/ml. Hormonal assay showed elevations of prolactin and oxytocin level (P ≤0.05).This study suggest the relationship between maternal stress and breastfeeding.

Amy C. H., Annessa S. K., Vanessa R. L., Aaron B. C. (2024). Oxytocin: physiology, pharmacology, and clinical application for labor management. American journal of obstetrics and gynecology Agata F. G., Katarzyna L., Paulina P., Magdalena K., Iwona S. K., Marta D. W. (2023). The involvement of prolactin in stress-related disorders. International Journal of Environmental Research and Public Health, 20(4), 3257 Athanasiou, N., Bogdanis, G. C., & Mastorakos, G. (2023). Endocrine responses of the stress system to different types of exercise. Reviews in Endocrine and Metabolic Disorders, 24(2), 251-266. Chantal L. (2005). Effects of stress on lactation. Pediatric Clinics of North America, 48(1), 221-234 Fatma Wati, Berliana M I., Hafidza A. (2022). Literature review: The relationship of anxiety towards the production of breast milk in postpartum mothers. Journal Research of Social Science, Economics, and Management, 11(1), 1932-19440. Hirotaka S., Tsuyoshi M., (2019). Differential effects of stress exposure via two types of restraint apparatuses on behavior and plasma corticosterone level in inbred BALB/cAJcl mice. Neuropsychopharmacology Reports, 40(1), 73-84. Jerome F., Robert L. B., Anuja D., Carmen J. W., Zev S. W. (2023). Yen & Jaffe’s reproductive Endocrinology-E-Book: Physiology, pathophysiology, and clinical management. Elsevier health sciences. Koolhaas J. M., Bartolomucci A., Buwalda B., S. F. de Boer. G. Fluggue, Korte S. M., Meerlo P., Murison R., Oliver B., Palanza P., Richter-Levin G., Sgoifo A., Steimer T., Stiedl O., G. van Dijk, M. Wohr, E. Fuchs (2011). Stress revisited: A critical evaluation of the stress concept. Neuroscience & Behavioral Reviews, 32(5), 1291-1301. LibreTexts (2023). Physiology of lactation. Retrieved from https://med.libretexts.org/Bookshelves/Anatomy_and_Physiology/Anatomy_and_Physiology_((Boundless)/27 3A_Human_Development_and_Pregnancy/27.8 3A_Lactation/27.8 3A_Physiology_of_Lactation Mustafa Al-Chalabi, Autumn N. B., Ihsan A. (2023). Physiology, prolactin. StatPearls: National Library of Medicine; National Center for Biotechn Perani, C. V. (2014). Peripartum Hypothalmus-Pituitary-Adrenal axis plasticity and its prevention by high-fat diet intake: Focus on the adrenal gland (Doctoral dissertation). Ranabir, S., & Reetu, K. (2011). Stress and hormones. Indian journal of endocrinology and metabolism, 15(1), 18-22. Stefanaki, C., Pervanidou, P., Boschiero, D., & Chrousos, G. P. (2018). Chronic stress and body composition disorders: implications for health and disease. Hormones, 17, 33-43. Torner, L., & Neumann, I. D. (2002). The brain prolactin system: involvement in stress response adaptations in lactation. Stress, 5(4), 249-257.