Isolation, Characterisation and Assessment of Antimalarial Properties of Khaya senegalensis (Stem back) extract

DOI: https://doi.org/10.33003/jobasr-2023-v1i1-19

Daskum A. M.

Abstract
In active malaria transmission areas, the disease is responsible for the death of many children less than five years of age and expecting mothers. Locals in underdeveloped African nations relied heavily on traditional herbal formulations to treat a number of ailments including malaria.In this study, the antimalarial potential of the stem back of African Mahogany (K. senegalensis) popularly known as Mad’aciinmost parts ofHausa land in Northern Nigeriawas further assessed with a view to substantiate traditional claim and revalidate already existing studies. The study also demonstrates the efficacies of crude Khaya senegalensis (Stem back) extracts on P. falciparum (3D7) in vitro. Secondary metabolites such as Alkaloids and Flavonoids were identified in all solvent extracts, the presence of which, might be indicative of the antimalarial activities observed. Similarly,suppression of parasite growth was observed to be dependenton the dosage used for all solvent extracts. Suppression of parasite growth was observed to be 52.97% for the highest concentrations (50 μg/mL) of hexane and methanolic extracts and 64.43% for the aqueous extracts. For the lowest concentrations (6.25 μg/mL) of each crude extract (Hexane = 41.32%, Methanol = 24.57% and Aqueous = 46.00%) respectively, suppression of growth of parasites was relatively lower compared to other dose levels. Further fractionation of crude extracts and assessment of antimalarial activities in other strains ofP. falciparum as well as curative and repository activities of the extracts and fractions on other malaria parasites in experimental animal models are required to substantiate the antimalarial efficacies of Khaya senegalensis.
References
Adebayo, J. O. and Krettli, A. U. (2011). Potential antimalarials from Nigerian plants: A review. Journal of Ethnopharmacology, 133: 289-302. Adebayo, J. O., Yakubu, M. T., Egwim, E. C., Owoyele, V. B. and Enaibe, B. U. (2003). Effect of ethanolic extract of Khaya senegalensis on some biochemical parameters of rat kidney. Journal of Ethnopharmacology, 88(1): 69- 72. Alain, K. Y., Oronce, D. O., Boniface, Y., Mhudro, Y., Pascal, A. D., Paul, T. F. et al. (2014). Free radical scavenging and antibacterial potential of two plants extracts (Khaya senegalensis and Pseudocedrela kotschyi) used in veterinary pharmacopoeia in Benin. Elixir Applied Chemistry, 76: 28720-28726. Amir, A. B. (2016). Establishment of a New Line of Plasmodium knowlesi. Univerity of Malaya, Parasitology. Kuala Lumpur, Malaysia: Unpublished PhD Thesis. Aguoru , C. U., Bashayi, C. G. and Ogbonna, I. O. (2017). Phytochemical profile of stem bark extracts of Khaya senegalensis by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Journal of Pharmacognosy and Phytotherapy, 9(3): 35-43. Basco, L. K. (2007). Field application of in vitro assays for the sensitivity of of human malaria parasites to antimalarial drugs. Geneva: World Health Organization. Bello, I. S., Oduola, T., Adeosun, O. G., Omisore, N. A., Raheem , G. O. and Ademosun, A. A. (2009). Evaluation of antimalarial activity of various fractions of Morinda lucida leaf extract and Alstonia boonei stem Bark. Global Journal of Pharmacology, 3 (3): 163-165. Bukar, A., Mukhtar , M. D. and Hassan, A. S. (2009). Phytochemical screening and antibacterial activity of leaf extracts of Senna siamea (lam) on Pseudomonas aeruginosa. Bayero Journal of Pure and Applied Sciences, 2 (1): 139-142. Chessed, G., Daskum, A. M., Maitandu, M. J., & Mustapha Tijjani. (2023). Hemin Polymerization Inhibitory Activities (HPIA) andin vitroAntimalarial Properties of CrudeSenna siameaLeaves Extract onP. falciparum, 3D7 strain. FUDMA Journal of Sciences, 7(1), 67 - 72. Daskum, A. M., Chessed, G., & Qadeer, M. A. (2019). Antiplasmodial efficacy of crude hexane, methanol and Aqueous Moringa oleifera Leaves extract on Chloroquine Sensitive Plasmodium falciparum (3D7). Trends in Basic Applied Sciences (p. 81). Kano: Bayero University. D’Alessandro, S., Silvestrini, F., Dechering, K., Corbett, Y., Parapini, S., Timmerman, M. et al. (2013). A Plasmodium falciparum screening assay for anti- gametocyte drugs based on parasite lactate dehydrogenase detection. Journal of Antimicrobial Chemotherapy, 68, 2048-2058. Donkor, A.-M., Oduro-Mensah, D., Ani, E., Ankamah, E., Nsiah , S., Mensah, D. E. et al. (2015). In vitro anti- plasmodial activity of aqueous and ethanolic extracts of Moringa oleifera and Phyllanthus amarus. International Journal of Biological Chemistry, 9(4): 198-206. Flannery, E. L., Chatterjee, A. K. and Winzeler, E. A. (2013). Antimalarial Drug Discovery: Approaches and Progress towards New Medicines. Nature Reviews Microbiology, 849-863. Huy Vu, Q., Van, H. T., Tran, V. T., Huynh, T. P., Nguyen, V. C., & Thanh Le, D. (2021). Development of a robust blood smear preparation procedure for external quality assessment. Practical Laboratory Medicine. doi:10.1016/j.plabm.2021.e00253 Ibrahim, J. A., Ayodele, E. A., Jegede, A. I. and Kunle, Y. F. (2006). Comparative studies on Khaya. A. Juss (Meliaceae) in Nigeria. African Journal of Biotechnology, 5 (11): 1154-1160. Ibrahim, M. A., Koorbanally, N. A. and Shahidul Islam, M. D. (2014). Antioxidative activity and inhibition of key enzymes linked to type-2 diabetes (α-glucosidase and α- amylase) by Khaya senegalensis. Acta Pharmacologica, 64: 311–324. Idowu, O. A., Soniran, O. T., Ajana , O. and Aworinde, D. O. (2010). Ethnobotanical survey of antimalarial plants used in Ogun State, Southwest Nigeria. African Journal of Pharmacy and Pharmacology, 4 (2): 55-60. Idowu, E. T., Ajaegbu, H. C., Omotayo, A. I., Aina, O. O. and Otubanjo, O. A. (2015). In vivo anti-plasmodial activities and toxic impacts of lime extract of a combination of Picralima nitida, Alstonia boonei and Gongronema latifolium in mice infected with chloroquine-sensitive Plasmodium berghei. African Health Sciences, 15 (4): 1262-1270. Kankia, H. I. and Zainab, S. A. (2015). Phytochemical analysis and antimicrobial activity of methanolic and ethanolic leaves, barks and roots crude extracts of Khaya senegalensis.International Journal of Scientific and Research Publications, 5 (1): 1-6. Kolawole, S. O., Kolawole , O. T. and Akanji, M. A. (2011). Effects of aqueous extract of Khaya senegalensis stem bark on biochemical and hematological parameters in rats. Journal of Pharmacology and Toxicology, 6 (6): 602-607. Kumar, S. R., Venkateshwar, C., Samuel, G. and Rao, G. S. (2013). Phytochemical screening of some compounds from plant leaf extracts of Holoptelea integrifolia (Planch.) and Celestrus emarginata (Grah.) used by Gondu tribes at Adilabad District, Andhrapradesh, India. International Journal of Engineering Science Invention, 2 (8): 65-70. Kunle , F. O., Ali, A. A. and Egharevba, H. O. (2013). Medicinal Plants Used for the Treatment of Malaria in Rukuba, Bassa Local Government Area of Plateau State, Nigeria. International Journal of Basic and Applied Sciences, 2 (4): 134-138. Lawal, B., Shittu, O. K., Kabiru, A. Y., Jigam, A. A., Umar, M. B., Berinyuy, E. B. et al. (2015). Potential antimalarials from African natural products: A review. Journal of Intercultural Ethnopharmacology, 4 (4): 318- 343. Matthews, H., Idris, M. U., Khan, F., Read, M. and Nirmalan, N. (2013). Drug repositioning as a route to anti-malarial drug discovery: preliminary investigation of the in vitro anti-malarial efficacy of emetine dihydrochloride hydrate. Malaria Journal. doi:http://www.malariajournal.com/content/12/1/359 Mojarrab, M., Shiravand, A., Delazar, A. and Afshar, F. H. (2014). Evaluation of in vitro antimalarial activity of different extracts of Artemisia aucheri Boiss. and A. armeniaca Lam. and fractions of the most potent extracts. The Scientific World Journal, 1-6. Muhammad, Y., Suleiman , M. M., Umar, A. M., Ahmed, G., Gumel, M. A., Mohammed, H. et al. (2017). Effect of crude methanol extract of Senna occidentalis on haematological parameters of wistar rats experimentally infected with Trypanosoma congolense. Journal of Pharmacognosy and Phytochemistry, 6 (5): 1217-1224. Odugbemi, T. O., Akinsulire, O. R., Aibinu, I. E., and Fabeku , P. O. (2007). Medicinal plants useful for malaria therapy in Okeigbo, Ondo State, Southwest Nigeria. African Journal of Traditional, Complementary and Alternative Medicines, 4 (2): 191- 198. Okokon, J. E., Antia, B. S., Azare, B. A. and Okokon, P. J. (2016). Antiplasmodial activity and cytotoxicity of ethanol extract of Zea mays root. Avicenna Journal of Phytomedicine, 1-10. Oliveira, A., Dolabela, M. F., Braga, F. C., Rose, J. L., Varotti, F. P. and Póvoa, M. M. (2009). Plant-derived antimalarial agents: new leads and efficient phythomedicines. Part I. Alkaloids. Annals of the Brazilian Academy of Sciences, 81 (4): 715-740. Onu, A., Saidu, Y., Ladan, M. J., Bilbis, L. S., Aliero, A. A. and Sahabi, S. M. (2013). Effect of Aqueous Stem Bark Extract of Khaya senegalensis on Some Biochemical, Haematological, and Histopathological Parameters of Rats. Journal of Toxicology, 1-9. Orman, E., Addo, P., Ofori, M. F. and Adosraku, R. K. (2015). Investigating the in-vivo Antiplasmodial Properties of Aqueous Extract of Moringa oleifera Lam (Moringaceae) Leaves. British Journal of Pharmaceutical Research, 5 (6): 419-430. Orwa, C., Mutua, A., Kindt, R., Jamnadass, R. and Anthony, S. (2009). Agroforestree Database: a tree reference and selection guide version 4.0. Kenya: World Agroforestry Centre. Pandey, S., Pandey, R. and Singh, R. (2014). Phytochemical Screening of Selected Medicinal Plant Cinnamon zeylanicum bark extract; Uttarakhand, India. International Journal of Scientific and Research Publications, 4(6): 1-6. Rasoanaivo, P., Wright, C. W., Willcox , M. L. and Gilber, B. (2011). Whole plant extracts versus single compounds for the treatment of malaria: synergy and positive interactions. Malaria Journal, 10: 4212-4222. Sabbatani, S., Fiorino, S. and Manfredi, R. (2010). The emerging of the fifth malaria parasite (Plasmodium knowlesi). A public health concern? Brazilian Journal of Infectious Diseases, 14 (3): 299-309. Senguttuvan, J., Paulsamy, S. and Karthika, K. (2014). Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pacific Journal of Tropical Biomedicine, 4 (1): 359-367. Shayoub, M. A., Ahmed, S. K., Sara, M. A., Asma , N. M., Ali , M. E., Ahmed., M. A. et al. (2016). Anti- malarial activity of Khaya senegalensis. Indo American Journal of Pharmaceutical Research, 6 (8): 1-5. Somsak, V., Borkaew, P., Klubsri, C., Dondee, K., Bootprom, P. and Saiphet, B. (2016). Antimalarial Properties of Aqueous Crude Extracts of Gynostemma pentaphyllum and Moringa oleifera Leaves in Combination with Artesunate in Plasmodium berghei- Infected Mice. Journal of Tropical Medicine. http://dx.doi.org/10.1155/2016/8031392 Stevens, G. C., Baiyeri, K. P. and Akinnnagbe, O. (2013). Ethno-medicinal and culinary uses of Moringa oleifera Lam. in Nigeria. Journal of Medicinal Plants Research, 7 (13): 799-804. Tona, L., Ngimbi, N. P., Tsakala, M., Mesia, K., Cimanga, K., Apers, S. et al. (1999). Antimalarial activity of 20 crude extracts from nine African medicinal plants used in Kinshasa, Congo. Journal of Ethnopharmacology, 68: 193–203. Trager, W. and Jenses, J. B. (1976). Human Malaria Parasites in Continuous Culture. Science, 193 (4254): 673-675. White, N. J., Pukrittayakamee, S., Hien, T. T., Abul Faiz, M., Mokuolu, O. A. and Dondorp, A. M. (2014). Malaria. The Lancet, 383: 723-735. Yusuf, A. Z., Zakir , A., Shemau, Z., Abdullahi, M. and Halima , S. A. (2014). Phytochemical analysis of the methanol leaves extract of Paullinia pinnata Linn. Journal of Pharmacognosy and Phytotherapy, 6(2): 10-16.
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