The skin is the largest organ interfacing with the external environment. The aim of the study is to compare skin microbiome of the following sites: Oily (eg Forehead, Nose and Chin), Moist (eg Elbow, breast, groin) and Dry (eg, arms, hands, and Abdomen) of male and female subjects in Obong University and its environs. The objectives of the study are to isolates, characterized and identify bacteria populations on human skin. The human skin though sterile prenatally becomes besieged by microorganisms. These microorganism comprising bacteria, fungi, protozoa and virus.A total of 54 skin swabs, 30 from male and 24 from female were collected from eighteen (18) subjects, 10 male and 8 female subjects and were analysed using standard microbiological methods. Five (5) bacterial isolates, Staphylococcusaureus, Staphylococcus epidermidis, Klebsellapneumonia, Pseudomonasaeruginosa and Cutibacterium were isolated from the representative skin sites .S.aureus and S.epidermidis dominated the moist and dry skin sites at 31% and 29%, 37% and 36%, respectively, while C.acnes occurred at 57% on the sebaceous sites. Based on gender differences, the isolates obtained from the female subjects from the three study sites showed S.aureus, S.epidermidis. C.acnes, P.aeruginosa and K.pneumonia occurring at the percentage prevalence of 35%, 36%, 39%, 39% and 36% respectively for the females and consequently at 65%, 64%, 61%, 61% and 64% for the male subjects. Based on age differences, the following bacteria, S.aureus, S.epidermidis, C.acnes, P.aeruginosa, and K.pneumonia were prevalent at 20%, 17%, 21%, 22%, and 20%., for the female between the age of 12-19 years and 22%, 17%, 23%, 20% and 18% for the males subjects between the age of 1019years. The increase in Staphylococcus aureus in men than women is attributed to the fact that men have more sweat glands than women (Kawahata, 1960).

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