Antibiogram Profiles of Heterotrophic Bacteria and Coliforms Isolated from Orogodo River in Agbor - Southern Nigeria
Jaboro A. G.
Oyem M. I.
Obayagbona O. N
Igborgbor J. C.
Konyeme T. E.
Oghene U. J.
Ikechukwu C.
Abstract
This study examined selected bacteriological attributes as well as the antibiogram and plasmid profiling of identified surface water borne bacterial cultures. Several water samples were collected from 3locations along the Orogodo River in Delta State, Nigeria. Using serial dilution and pour plate procedures,the mean total heterotrophic bacterial (THBC) and coliform counts (TCC) of the water samples were determined. Tentative identification of the bacterial isolates was achieved by subjecting the respective isolates to several biochemical tests which included Gram staining and catalase production tests. The overall mean THBC and TCC ranged from 3.59 ± 241 × 10⁵cfu/ml to 7.06 ± 177 × 10⁵ cfu/ml and 3.32 ± 79 × 10⁴ cfu/ml to 4.85 ± 186 × 10⁴cfu/ml respectively. The observed difference between the overall mean THBC and TCC were notinsignificant (P> 0.05). The tentatively identified bacterial isolates were; Bacillus sp., Weisselasp.,Pseudomonas sp., Corynebacterium sp., Micrococcussp.,Pseudomonassp.,Shigellasp.,Serratia sp., Klebsiella sp., Eschericia coli and Citrobacter sp. respectively. Antibiotic susceptibility/resistance testing of the bacterial isolates revealedthat bothCorynebacterium sp. and Serratia sp. Exhibitedmaximal multiple drug resistance index (MDRI) value of 0.6 respectively, while Citrobacter sp. showed the lowest MDRI value (0.1). Plasmid profiling indicated that Bacilllussp, Weisselasp., Corynebacterium sp., Micrococcus sp. and Shigellaspharbored plasmids while no plasmidwas detected in Klebsiella sp. AndE. coli respectively.Findings from this study suggested an urgent need for continuous monitoring of the river as well as implementation of pollution control strategies to protect bothpublic health and the Orogodo river aquatic ecosystem respectively.
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