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Prevalence and antibiotic susceptibility pattern of Streptococcus pneumoniae amongst residents of Obong Ntak in Etim Ekpo Local Government of Akwa Ibom State, Nigeria

DOI: https://doi.org/10.33003/jobasr-2023-v1i1-19

Anosike.

I. K.

Egbomuche.

R. C.

Etang.

U. E.

Akpan.

N. G.

Abasiubong.

V. N.

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A Publication of the Faculty of Physical Sciences, Federal University Dutsin-Ma, Dutsin-Ma, Katsina State, Nigeria.
Abstract
Streptococcus pneumonia is among the major causative agents of respiratory tract infections and colonizes the nasopharyngeal cells of asymptomatically healthy individuals. Prevalence and antibiotics sensitivity of Streptococcus pneumonia amongst residents in a rural setting was evaluated. A total of 250 nasal swab samples (125 males and 125 females) were collected using sterile swab sticks. Administration of open-ended questionnaires, culture of the samples, identification of isolates, antimicrobial sensitivity test and determination of prevalence rate were all done using standard protocols. Out of the 250 samples collected, 35 were positive for S. pneumonia giving an overall prevalence of 14%. Based on sex, the prevalence rates were 15% and 20% for males and females, respectively. Prevalence rates of 12.5%, 13.7%, 12.7% and 25% were recorded for age groups of 10-20, 21-40, 41-60 and >60 years, respectively. The presence of S. pneumoniae based on the health status of the participants showed that the prevalence rates were 20% and 11.70% for clinically ill and clinically healthy individuals, respectively. The resistance profile to the test antibiotics were 22.9%, 22.9%, 11.4%, 100%, 100%, 34.3%, 34.3%, 100%, 54.3% and 77.1% respectively to perfloxacin, septrin, ciprofloxacin, amoxacilin, zinacef, gentamicin, erythromycin, ampliclox, streptomycin and rocephin. Ciprofloxacin was the most effective antibiotic followed by perfloxacin and septrin. Our isolates showed 100% multi-drug resistance and is a cause for concern.
References
Arai, J., Hotomi, M., Hollingshead, S. K., Ueno, Y., Briles, D. E. &Yamanaka, N. (2011). Streptococcus pneumoniae isolates from middle ear fluid and nasopharynx of children with acute otitis media exhibit phase variation. Journal of Clinical Microbiology,49, 1646-1649. Bastiaens, G. J. H., Cremers, A. J. H., Coolen, J. P. M.,Nilesen, M. T., Boeree, M. J., Hopman, J & Wertheim, H. F. L. (2018). Nosocomial outbreak of multi-resistant Streptococcus pneumoniae serotype 15A in a centre for chronic pulmonary diseases.Antimicrob Resistance Infect Control,7,158 (2018). https://doi.org/10.1186/s13756-018-0457-3 Bauer,A. W., Kirby, W. M. N., Sherries, J. C. &Turck, M. (1960). Antibiotics susceptibility testing by a Standardized single disk method. American Journal of clinical pathology,36, 493-496. Bogaert, D., De Groot, R. &Hermans, P. W. M. (2004).Streptococcuspneumoniae colonization: the key to pneumococcal disease. Lancet Infectious Disease, 4, 144- 154. Büyükcam, A.,Güdücüoğlu, H., Karaman, K., Gürbüz, V., Aliyev, E., Kara, A.&Ceyhan, M. (2017). Invasivepneumococcal infection due to serotype 15A after the pneumococcal conjugate vaccine implementation in Turkey.Human Vaccine Immunotherapy,13(8), 1892-1894. Cheesbrough, M. (2009). Medical laboratory manual for tropical countries, microbiology (ELBS), New York. 2nd edition, pp. 62-70. Chenoweth, C. E. (2009)."Streptococci." APIC Text of Infection Control and Epidemiology. Washington, D.C. Association for Professionals in Infection Control and Epidemiology, 71, 1-71. Clinical Laboratory Standard Institute (2012). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-second Informational Supplement. Clinical Laboratory Standard Institute. Wayne, Pennsylvania, USA Magiorakos. Crossley, K. B. & Lippincott, W. W. (2004). “Streptococci” Hospital Epidemiology and Infection Control. Third Edition Philiadelphia. Pp. 523-524. Emele, F. E. (2000). Etiologic spectrum and pattern of antimicrobial susceptibility in bacterial meningitis in Sokoto, Nigeria. Acta Paediatric,89(8), 942-6. Falade, A. G., Lagunju, I. A., Bakare, R. A., Odekanmi, A. A. &Adegbola, R. A. (2009).Invasive pneumococcal disease in children aged, 5 years admitted to 3 urban hospitals in Ibadan, Nigeria. Clinical Infection Disease,48, 190-6. Fang, G. D., Fine, M., Orloff, J.&Arisumi D. (1990). New and emerging etiologies for community-acquired pneumonia with implications for therapy: A prospective multicenter study of 359 cases. Medicine (Baltimore), 69(5), 307-16. Garba, I., Abdulrazaq, G. H., Aminu, B. M. & Mohammad, M. B. (2015). Epidemiology and Clinical Outcomes of Community Acquired Pneumococcal Infection in North-West Nigeria. Sub-Saharan African Journal of Medicine,2,79-84. Habib, A. G., Nwokedi, E. E., Ihesiulor, I. U., Mohammed, A. & Habib, Z. G. (2003). Widespreadantibiotic resistance in savannah Nigeria. African Journal Medical of Science,32, 303-305. Holt et al (1995). Bergey’s manual of determinative bacteriology. 9th Edition, Maryland, Iliyasu, G., Habib, A. G. & Ahmed, M. (2011). Clinical pattern and profile of invasive pneumococcal infection in Aminu Kano Teaching Hospital. National Postgraduate Medical College Nigeria. Pneumococcal infection in Nigeria: Preparing for the vaccine. SubSaharan African Journal of Medicine vaccine, 1,15-19. Kim, P. E., Musher, D. M., Glezen, W. P., RodriguezBarradas, M. C., Nahm, W. K. & Wright, C. E. (1996). Association of invasive pneumococcal disease with season, atmospheric conditions, air pollution, and the isolation of respiratory viruses. Clinical Infectious Diseases,22, 100-106. Kraemer, S. A., Ramachandran, A., & Perron, G. G. (2019). Antibiotic Pollution in the Environment: From Microbial Ecology to Public Policy. Microorganisms, 7(6), 180. https://doi.org/10.3390/microorganisms7060180 Lindsay, K., Lesley, M. G., Sara, T. &Bernard, B. (2016). Biological and Epidemiological Features of AntibioticResistant Streptococcus pneumoniae in Pre- and PostConjugate Vaccine Eras: A United States Perspective. Clinical Microbiology Reviews,29(3), 525-552. Mandell, G. L., Gordon, D. R., John, E. B. & Raphael, D. (2010). "Streptococcus Pneumoniae." Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases, 7th ed, New York: Elsevier/Churchill Livingstone.2010 Martson, B. J., Plouffe, J. F., File, T. M.,et al. (1997). Incidence of Community-acquired pneumonia requiring hospitalization: results of population based active surveillance in Ohio.he Community-Based Pneumonia Incidence Study Group.Archines Internal Medicine, 157, 170-918. Musa, B. M., Tijjani, B. M., Okpapi, J. U., Borodo, M. M., Babashnai, M. & Shehu, Y. (2008). et al. Bacterial isolates and Antibiotic Sensitivity in Community Acquired Pneumonia. Nigeria Medical Journals, 49, 63- 66. Nakano, S., Fujisawa, T., Ito, Y., Bin, C, et al. (2019). Whole-genome sequencing analysis of multidrugresistant serotype 15A Streptococcus pneumoniae in Japan and the emergence of a highly resistant Serotype 15A-ST9084 clone.Antimicrobial Agents Chemotherapy, 63(5), e02579-18. Ojo, M. O. (2009). Manual of Pathogenic Bacteria and Fungi. Banola Multi Project Limited. P.O. Box19927 University of Ibadan Post Office, Ibadan, Nigeria.2009. Okonkwo, E. E. & Oguamanam, C. C. (2013). Traditional Crafts and Tourism Development and Promotion in Etim Ekpo Local Government of Akwa Ibom State, Nigeria. Research on Humanities and Social Sciences, 3(6), 139- 147. Ozumba UC. Changing pattern of acute bacterial meningitis in Enugu, Nigeria. East Africa Medical Journal 1994; 71: 300-303. Revathy, A., Hema, R., Rohaidah, H., Fairuz, A., Nazirah, S.&Norazah, A. (2019). Multidrug-resistant Streptococcus pneumoniae causing invasive pneumococcal disease isolated from a paediatric patient. International Journal of Infectious Diseases 2019; 90: 219-222. Scott, J. A. G. (2007). The preventable burden of pneumococcal disease in the developing world. Vaccine, 25, 2398-2405. Setia, M. S. (2016). Methodology Series Mdoule 3: Cross Sectional Studies. Indian Journal of Dermatology, 61(3), 261-264. Sham, D. F., Thomsberry, C, Mayfield, D. C., Jones, M. E. &Karlowsky, J. A. (2001). Multidrug resistant urinary tract isolates of Escherichia coli: Prevalence and patient demographics in united states in 2000. Antimicrob Agents Chemother, 45(5), 1402-1406. Sleeman, K. L., Daniels, L., Gardiner, M., Griffith, D., Deeks, J. J. &Dagan, R. (2005). Acquisition of Streptococcus pneumoniae and nonspecific morbidity in infants and their families: A cohort study. Pediatrics Infectious Disease Journal, 24, 121-127. Srinivasan, A. P., Carey, A., Carmeli, R. B., Falagas, Y. M. E.,et al. (2012). Multidrug resistance, extensively drug resistance and pandrug-resistance bacteria: An international expert proposal for interim standard definations for acquired resistance. Clin Microbiol Infection, 18, 268-281. Toda, H., Satoh, K. & Komatsu, M. (2018). Laboratory surveillance of antimicrobial resistance and multidrug resistance among Streptococcus pneumoniae isolated in the Kinki regionof Japan - Comparison of the prevalence of drug-resistant strains before and after introduction of conjugated pneumococcal vaccine. Journal of Infection and Chemotherapy, 24, 171-176. Wang, C. Y., Chen, Y. H., Fang, C., et al (2019). Antibiotic resistance profiles and multidrug resistance patterns of Streptococcus pneumoniae in pediatrics. Medicine, 98(24), 1-7. Weiser, J. N., Ferreira, D. M. & Paton, J. C. (2018). Streptococcus pneumoniae: transmission, colonization and invasion. Nat Rev Microbiol. 16(6), 355-367. doi: 10.1038/s41579-018-0001-8. World Health Organization (2007). Pneumococcal conjugate vaccine for childhood immunization-WHO position paper. Weekly Epidemiological Records82, 93- 104. World Health Organization (2022). Pneumonia. https://www.who.int/news-room/factsheets/detail/pneumonia. Accessed 20/05/2022.
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