Assessment of Plants Alkaloids Isolated from Calotropis Procera for Managing Pod Sucking Bug, Clavigralla tomentosicollis Stal (Hemiptera: Coreidae) Infestation on Cowpea

DOI: https://doi.org/10.33003/jobasr-2023-v1i1-19

Audi, A. H.

Wada, Y. B

Abstract
Crude plant extracts used to control insect pests are less specific and the active compounds may have antagonistic effects. The trials were thus conducted to assess the effectiveness of alkaloids from Calotropis procera in controlling the pod sucking bug Clavigralla tomentosicollis on cowpea. Three cowpea varieties, Ife brown, IT07K-318-33, and IT07K-292-10, were grown in beds assigned to four treatment groups: 500µg/ml, 250µg/ml, 125µg/ml, and 0µg/ml of the extracts as a control. Each treatment was replicated three times in a randomized complete block design. The findings revealed a significant decrease (p<0.05) in the population of C. tomentosicollis in the treated plots compared to the control. The highest level of protection was observed in plots sprayed with 500µg/ml and 250µg/ml, with no significant difference (p>0.05) between treatment and spray regimes (1-week after spray (1-WAS) and 2-weeks after spray (2-WAS). The average percentage of pod damage was significantly lower at higher treatment concentrations (500µg/ml and 250µg/ml) compared to lower concentrations and the control. There was no significant difference in pod damage among the three cowpea varieties. The relative adult mortality of C. tomentosicollis followed a similar pattern, with higher concentrations (500µg/ml and 250µg/ml) resulting in a significant decrease in ITOK-292-10 compared to other varieties. In the ITOK7- 318-33 variety, there was no significant difference in mortality rates between treatments (500µg/ml, 250µg/ml, and 125µg/ml) and spray regimes. This implied that treatment of cowpea ITOK-292-10 with the higher concentration of the Alkaloid (2-piperidinone, N-(4-bromo-n-butyl) would yield an optimum level control of the notorious bug.
References
Agbogidi, O. M. and Egho, E. (2012). Evaluation of eight varieties of cowpea ( Vigna unguiculata ( L .) Walp ) in Asaba agro-ecological environment , Delta State , Nigeria. European Journal of Sustainable Development, 1(2), 303–314. Bajpai, V. K., Majumder, R. and Park, J. G. (2016). Isolation and purification of plant secondary metabolites using column-chromatographic technique. Bangladesh Journal of Pharmacol, 2(5), 844–848. https://doi.org/10.3329/bjp.v11i4.28185 Degri, M. M., Mailafiya, D. M., and Wabekwa, J. W. (2013). Efficacy of aqueous leaf extracts and synthetic insecticide on pod-sucking bugs infestation of cowpea (Vigna unguiculata (L.) Walp) in the Guinea Savanna Region of Nigeria. Advances in Entomology, 1(2), 10–14. .doi.org/10.4236/ae.2013.12003 Advances Devender, R. and Ramakrishna, H. (2017). GC-MS analysis of bioactive compounds in methanolic extract of Leucas lavandulaefolia Rees - A potential folklore medicinal plant. Journal of Pharmacognosy and Phytochemistry, 6(4), 405–406. doi.org/6-3-134-520 Doumandji-Mitiche, A. and Bahi, F. (2013). Insecticidal activity of alkaloids extract of Pergularia tomentosa (Asclepiadaceae) against fifth instar larvae of Locusta migratoria (Fabricius 1781) (Orthoptera : Acrididae). International Journal of Science and Advanced Technology, 3(6), 8–9. http://www.ijsat.com Emmanuel, E., Ching F. P. Abiodun, F. and Sunday A. A. (2013). Cola Rostrata: Phytochemical and Toxicity Studies . Journal of Applied Science and Environmental. Management, 17 (4) 603-607. http://dx.doi.org/10.4314/jasem.v17i4.19 Ge, Y., Liu, P., Yang, R., Zhang, L., Chen, H., Camara, I. and Shi, W. (2015). Insecticidal Constituents and Activity of Alkaloids from Cynanchum mongolicum. 17483– 17492. https://doi.org/10.3390/molecules200917483 Gujjeti, R. P. and Mamidala, E. (2013). Phytochemical Screening and Thin Layer Chromatographic Studies of Aerva lanata Root Extract. International Journal of Innovative Research in Science, Engineering and Technology, 2(10), 5725–5730. www.ijirset.com Haydelba D’Armas V.V. and Ordaz, G. (2018). Phytochemical screening and bioactivity analysis of extracts from Helicteres guazumifolia Kunth (Sterculiaceae). International Journal of Herbal Medicine, 6 (1):47–50. www.florajournal.com James, R., Malcolm, K., Dariel, B. and Joanna, V. (2014). Using Soxhlet Ethanol Extraction to Produce and Test Plant Material (Essential Oils) for their Antimicrobial Properties. Journal of Microbiology and Biology Education, 15(1), 45-46. doi.org/10.1128/jmbe.v15i1.656 Kumar, C. P. and Sachin, J. (2013). Extraction and Isolation of Bioactive Compounds from. International Journal of Pharmaceutical Sciences Review and Research, 20 (2), 101–106. www.globalresearchonline.net Lucie, C., Martina, H., Andrea, K. , Nina, B. , Jakub, C., Daniel, C., Zdeněk, N., Kateřina, M., Jiří K., Kamil, K.C. and Lubomír O. (2013). Alkaloids from Chlidanthus fragrans and their Acetylcholinesterase, Butyrylcholinesterase and Prolyl Oligopeptidase Activities , Natural Product Communications, 8 (11) 1542-1544 doi /10.1177/1934578X1300801110 Oparaeke, A. M., Dike, M. C. and Amatobi, C. I. (2005). Botanical Pesticide Mixtures For Insect Pest Management On Cowpea , Vigna Unguiculata ( L .) Walp. The Pod Borer, Maruca vitrata Fab. (Lepidoptera : Pyralidae ) and Pod Sucking Bug , Clavigralla tomentosicollis Stal (Heteroptera : Coreidae.) Agricultura Tropica Et Subtropica, 38 (2), 33–38. cabidigitallibrary.org Perillo, A. C., Kucharik, C. J., Meehan, T. D., Serbin, S. P., Singh, A., Townsend, P. A., Gratton, C. (2015). Use of insect exclusion cages in soybean creates an altered microclimate and differential crop response. Agricultural and Forest Meteorology, 208 (5), 50–61. https://doi.org/10.1016/j.agrformet.2015.04.014 Rauf, F. M. A. and Sardar, M. A. (2011). Effect of crude seed extract of some indigenous plants for the control of legume pod borer (Maruca vitrata F.) on country bean. Bangladesh Journal of Agriculture, 36 (1): 41–50 doi: 10.5555/20123015676 Singh, M., Kumari, S., Attri, R. and Kumar, S. (2015). Impact of Calotropis procera Leaf Extracts on the Survival, Morphology and Behaviour of Dengue Vector, Aedes aegyptii L . Journal of Undergraduate Research and Innovation, 1(3), 96–107. journals.du.ac.in Steele, L. D., Sun, W., Valero, M. C., Adebayo, J., Mook, K., Coates, B. S., Pittendrigh, B. R. (2017). The Mitogenome of the Brown Pod-Sucking Bug Clavigralla Tomentosicollis Stäl (Hemiptera : Coreidae ). Agri Gene, 5, 27–36.http://dx.doi.org/10.1016/j.aggene.2017.07.002
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