Antibiosis of Salicylic acid Pre-Hardening Treatments of Cowpea on Development of Pod Sucking Bug, Clavigralla tomentosicollis Stal. (Hemiptera: Coreidae)

DOI: https://doi.org/10.33003/jobasr-2023-v1i1-19

Audi, A. H.

Mukhtar, F. B.

Abstract
Induced plant response using Exogenous Salicylic acid has a significant potential to control physiological interactions of Phytophagous insects The experiment was conducted at the University Research farm, Faculty of Agriculture Bayero University, Kano (11°58 ‘N, 8° 25’ E and 457m above sea level) to evaluate the effect of SA-treatment on Development of Pod sucking bug of cowpea. Four different cowpea varieties (IT97K-1069-6, IT98K-205-8, IT89KD-288 and Dan’ila) pre-hardened with Salicylic acid were established in various replicated field cages in completely randomized design. Five-pairs each of fresh bugs were introduced into the various cages. These were allowed for 2-weeks to mate and oviposit after which all adult insects are removed. Introduced insects showed variable developmental response to the different Pre-hardened cowpea varieties in SA hormones (P<0.001). Interactions of treatments and varieties was also found significant (P<0.001).Cowpea seeds treated with lower (5ppm) concentrations of SA show greater deleterious effects on the development of the pod bugs than the higher (10 & 20ppm) concentrations and the controls (Distilled water & Untreated seeds). Of the four Cowpea varieties screened, IT97K-1069-6 and IT89KD-288 of 5ppm SA-treatments show higher antibiosis on development of C. tommentosicollis, resulting to low oviposition (41.33 and 45.67), higher percentage nymphal mortalities (71.59% and 77.24%) and mean unhatched eggs of 27.33 and 32.00 respectively, with smaller adults bugs (3.55mg and 3.27mg) at emergence. Phytochemical screening of the treatments using GC-MS analysis showed high concentrations of cumene, eugenol and sesquiterpenes in the tolerant varieties. These relations should be explored extensively toward sustainable plant protection.
References
Aljbory1, Z and Chen, M.(2018). Indirect plant defense against insect herbivores: a review. Insect Science, 25 (1):2-23. doi: 10.1111/1744-7917.12436 Audi, A. H. and Mukhtar, F. B. (2019). Evaluation of Salicylic acid Pre-Hardening Treatments of Cowpea for Resistance against the Flea Beetle, Podagrica fuscicornis Linn. (Coleoptera: Chrysomelidae) International Journal of Environment, Agriculture and Biotechnology, 4 (1):33-40. Aviv, D. H., Rusterucci, C., Holt, B. F., Dietrich, R. A., Parker, J. E. and Dangl, J. L. (2002). Runaway cell death, but not basal disease resistance, in lsd1 is SA- and NIM1/NPR-dependent. Plant Journal, 29: 381–391. Berens, Matthias L, Berry, H. M., Mine, A., Argueso, C. T., Tsuda, K. (2017). Evolution of Hormone Signaling Networks in Plant Defense. Annual Review Phytopathology, 4:55:401-425 doi: 10.1146/annurev-phyto-080516-035544 Brodersen, P., Petersen, M., Pike, H. M., and Olszak, B. (2002). Knockout of Arabidopsis accelerated-cell-death11 encoding as phingosine transfer protein causes activation of programmed cell death and defense. Genes and Development, 16: 490–502. doi:10.1101/gad.218202 Darra, B. L., Seith, S. P., Singh, H. and Mendiratta, R. S. (1973). Effects of hormone directed presoaking emergence and growth of osmotically stressed wheat (Triticum aestivum L) -seeds. Agronomy Journal, 65: 295-299. doi:10.5958/2319-1198.2018.00022.2 De Boer, J. G., Posthumus, M. A. and Dicke, M. (2004). Identification of volatiles that are used in discrimination between plants infested with prey or nonprey herbivores by a predatory mite. Journal of Chemical Ecology, 30:22-30.bv doi: 0098-0331/04/1100-2215/0 Devi, C.B., Kushwaha, A. and Kumar, A. (2015). Sprouting characteristics and associated changes in nutritional composition of cowpea (Vigna unguiculata). Journal of Food Science Technology, 52 (10), 6821–6827. doi:10.1007/s13197-015-1832-1 Divol, F., Vilaine, F., Thibivilliers, S., Amselem J, and Palauqui, J. C. (2005). Systemic response to aphid infestation by Myzus persicae in the phloem of Apium graveolens. Plant Molecular Biology, 57: 517–540. doi/10.1007/s11103-005-0338-z Jackai, L.E.N., Nokoe, S., Tayo, B.O. and Koona, P. (2001). Interferences on pod wall and seed defenses against the brown cowpea coreid bug, Clavigralla tomentosicollis Stal. (Hem., Coreidae) in wild and cultivated Vigna species. Journal of Applied Entomology, 125, 277–286. doi: 10.1603/0022-0493-95.6.1281 Lortzing, V., Oberländer, J., Lortzing, T. Tohge, T., Steppuhn, A., Kunze, R., Hilker, M. (2019). Insect egg deposition renders plant defence against hatching larvae more effective in a salicylic acid‐dependent manner Plant Cell and Environment, 42 (3): 1019-1032 doi: 10.1111/pce.13447 Maffei, M.E., Mithofer, A. and Boland, W. (2007). Insects feeding on plants: rapid signals and responses preceding the induction of phytochemical release. Phytochemistry, 68:46- 59. doi.org/10.1016/j.phytochem.2007.07.016 Maleck, K. and Dietrich, R. A. (1999). Defense on multiple fronts: how do plants cope with diverse enemies? Trends Plant Science, 4: 215–219. doi:https://doi.org/10.1016/S1360-1385 (99)01415-6 Ofuya, T. I. and Osadahun, J. M. (2005). Effect of three Different Powders on Behaviour, Mortality and Reproductive Fitness of Callosobruchus maculatus (Coleoptera: Bruchidae). Zoological Research, 26(6):603- 608. Pancheva, T.V. and Popova, L. P., Uzunova, A. N. (1996). Effects of salicylic acid on growth and photosynthesis in barley plants. Journal of Plant Physiology, 149, 57–63. doi.org/10.1016/S0176-1617 (96) 80173-8 Pieterse, C.M.J. and van Loon, L.C. (1999). Salicylic acidindependent plant defence pathways. Trends in Plant Science, 4: 52–58. doi.org/PII: S1360-1385(98)01364-8 Singh, C. and Singh, N.N. (2015). Bioefficacy and economics of different insecticides again legume pod borer (Maruca vitrata Fabricius) infesting cowpea (Vigna unguiculata Walpers). Indian Journal of Agricultural Resources, 49 (4), 358–362. doi : 10.5958/0976- 058X.2015.00065.7 Sponsel, V. M. and Hedden, P. (2004). Gibberellin biosynthesis and inactivation of Plant hormone biosynthesis, signal transduction. Action. Davies P. J. Ed: Part B, Kluwer Academic. Publ. Dordrecht, 63-94pp. doi: 10.1104/pp.105.064162 Tanzubil, P. B. (2000). Field evaluation of Neem extracts for control of insect pests of cowpea in Northern Ghana. Journal of Tropical Forest Products (Malaysia) 6: 165 – 172. doi:10.5555/20013089506 Underwood, N., Rausher, M. D. and Cook, W. (2002). Bioassay versus chemical assay: measuring the impact of induced and constitutive resistance on herbivores in the field. Oecologia, (Berlin) 131:211–219. doi 10.1007/s00442-002- 0867-y Valls, J., Millán, S., Martí, M.P., Borràs, E. and Arola, L. (2009). Advanced Separation Methods of Food Anthocyanins, Isoflavones and Flavanols. Journal of Chromatography A, 1216, 43: 7143-7172. doi.org/10.1016/j.chroma.2009.07.030 Voster, I. H. J., Jansen, V. R. W., VanZijl, J. J. B. and Venter, S. L. (2007). The importance of traditional leafy vegetables in South Africa. African Journal of Food, Agric. Nutritional Development, 7(4): 1-12. doi: 10.5897/AJAR09.724
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